As this method relies on assumptions about particle shape and size distribution, it is inadvisable in counts of objects displaying an irregular shape and/or a highly variable size, such as β-atretic follicles or POC. 2000, Coward and Bromage, 2002 Medina et al., 2002, 2007 Murua et al., 2003 Cooper et al., 2005) is based on the equation developed by Weibel and Gomez (1962). ![]() So far, the most widely used stereological procedure to estimate fish oocyte numbers (Emerson et al., 1990 Greer Walker et al. The first practical stereological method was based on the Delesse’s principle, which states that the fractional volume of a component in a material is proportional to its fractional crosssectional area. So, stereological applications provide quantitative information about 3-D structures from measurements made on 2-D sections. Stereology is the science of the three-dimensional interpretation from bidimensional images. Estimates of fecundity in fishes can be obtained from counts of large yolked oocytes (potential fecundity) or postovulatory complexes (POC, =postovulatory follicles) (realized fecundity) applying stereological methods to histological sections of the ovaries.
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